Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A (2024)

De Donato, D. P., Effner, R., Nordengrün, M., Lechner, A., Darisipudi, M. N., Volz, T., Hagl, B., Bröker, B. M. (2024). Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A. Cytokine, 180, Article 156634. https://doi.org/10.1016/j.cyto.2024.156634

De Donato, D. P. ; Effner, R. ; Nordengrün, M. et al. / Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A. In: Cytokine. 2024 ; Vol. 180.

@article{c977a66b79fd4ab2ad7920796b79cda9,

title = "Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A",

abstract = "Background: Serine protease-like (Spl) proteins produced by Staphylococcus (S.) aureus have been associated with allergic inflammation. However, effects of Spls on the epidermal immune response have not been investigated. Objectives: To assess the epidermal immune response to SplA, SplD and SplE dependent on differentiation of keratinocytes and a Th2 or Th17 cytokine milieu. Methods: Human keratinocytes of healthy controls and a STAT3-hyper-IgE syndrome (STAT3-HIES) patient were cultured in different calcium concentrations in the presence of Spls and Th2 or Th17 cytokines. Keratinocyte-specific IL-8 production and concomitant migration of neutrophils were assessed. Results: SplE and more significantly SplA, induced IL-8 in keratinocytes. Suprabasal-like keratinocytes showed a higher Spl-mediated IL-8 production and neutrophil migration compared to basal-like keratinocytes. Th17 cytokines amplified Spl-mediated IL-8 production, which correlated with neutrophil recruitment. Neutrophil recruitment by keratinocytes of the STAT3-HIES patient was similar to healthy control cells. Conclusion: S. aureus-specific Spl proteases synergized with IL-17A on human keratinocytes with respect to IL-8 release and neutrophil migration, highlighting the importance of keratinocytes and Th17 immunity in barrier function.",

keywords = "Keratinocytes, Neutrophil migration, S. aureus, Serine protease-like proteins (Spls), STAT3-hyper IgE syndrome (HIES), Th2/Th17 immunity",

author = "{De Donato}, {D. P.} and R. Effner and M. Nordengr{\"u}n and A. Lechner and Darisipudi, {M. N.} and T. Volz and B. Hagl and Br{\"o}ker, {B. M.} and Renner, {E. D.}",

note = "Publisher Copyright: {\textcopyright} 2024 Elsevier Ltd",

year = "2024",

month = aug,

doi = "10.1016/j.cyto.2024.156634",

language = "English",

volume = "180",

journal = "Cytokine",

issn = "1043-4666",

publisher = "Academic Press Inc.",

}

De Donato, DP, Effner, R, Nordengrün, M, Lechner, A, Darisipudi, MN, Volz, T, Hagl, B, Bröker, BM 2024, 'Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A', Cytokine, vol. 180, 156634. https://doi.org/10.1016/j.cyto.2024.156634

Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A. / De Donato, D. P.; Effner, R.; Nordengrün, M. et al.
In: Cytokine, Vol. 180, 156634, 08.2024.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A

AU - De Donato, D. P.

AU - Effner, R.

AU - Nordengrün, M.

AU - Lechner, A.

AU - Darisipudi, M. N.

AU - Volz, T.

AU - Hagl, B.

AU - Bröker, B. M.

AU - Renner, E. D.

N1 - Publisher Copyright:© 2024 Elsevier Ltd

PY - 2024/8

Y1 - 2024/8

N2 - Background: Serine protease-like (Spl) proteins produced by Staphylococcus (S.) aureus have been associated with allergic inflammation. However, effects of Spls on the epidermal immune response have not been investigated. Objectives: To assess the epidermal immune response to SplA, SplD and SplE dependent on differentiation of keratinocytes and a Th2 or Th17 cytokine milieu. Methods: Human keratinocytes of healthy controls and a STAT3-hyper-IgE syndrome (STAT3-HIES) patient were cultured in different calcium concentrations in the presence of Spls and Th2 or Th17 cytokines. Keratinocyte-specific IL-8 production and concomitant migration of neutrophils were assessed. Results: SplE and more significantly SplA, induced IL-8 in keratinocytes. Suprabasal-like keratinocytes showed a higher Spl-mediated IL-8 production and neutrophil migration compared to basal-like keratinocytes. Th17 cytokines amplified Spl-mediated IL-8 production, which correlated with neutrophil recruitment. Neutrophil recruitment by keratinocytes of the STAT3-HIES patient was similar to healthy control cells. Conclusion: S. aureus-specific Spl proteases synergized with IL-17A on human keratinocytes with respect to IL-8 release and neutrophil migration, highlighting the importance of keratinocytes and Th17 immunity in barrier function.

AB - Background: Serine protease-like (Spl) proteins produced by Staphylococcus (S.) aureus have been associated with allergic inflammation. However, effects of Spls on the epidermal immune response have not been investigated. Objectives: To assess the epidermal immune response to SplA, SplD and SplE dependent on differentiation of keratinocytes and a Th2 or Th17 cytokine milieu. Methods: Human keratinocytes of healthy controls and a STAT3-hyper-IgE syndrome (STAT3-HIES) patient were cultured in different calcium concentrations in the presence of Spls and Th2 or Th17 cytokines. Keratinocyte-specific IL-8 production and concomitant migration of neutrophils were assessed. Results: SplE and more significantly SplA, induced IL-8 in keratinocytes. Suprabasal-like keratinocytes showed a higher Spl-mediated IL-8 production and neutrophil migration compared to basal-like keratinocytes. Th17 cytokines amplified Spl-mediated IL-8 production, which correlated with neutrophil recruitment. Neutrophil recruitment by keratinocytes of the STAT3-HIES patient was similar to healthy control cells. Conclusion: S. aureus-specific Spl proteases synergized with IL-17A on human keratinocytes with respect to IL-8 release and neutrophil migration, highlighting the importance of keratinocytes and Th17 immunity in barrier function.

KW - Keratinocytes

KW - Neutrophil migration

KW - S. aureus

KW - Serine protease-like proteins (Spls)

KW - STAT3-hyper IgE syndrome (HIES)

KW - Th2/Th17 immunity

UR - http://www.scopus.com/inward/record.url?scp=85194273263&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2024.156634

DO - 10.1016/j.cyto.2024.156634

M3 - Article

AN - SCOPUS:85194273263

SN - 1043-4666

VL - 180

JO - Cytokine

JF - Cytokine

M1 - 156634

ER -

De Donato DP, Effner R, Nordengrün M, Lechner A, Darisipudi MN, Volz T et al. Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A. Cytokine. 2024 Aug;180:156634. doi: 10.1016/j.cyto.2024.156634